Journal: Cell Death & Disease

Article Title: PA-MSHA induces inflamed tumor microenvironment and sensitizes tumor to anti-PD-1 therapy

doi: 10.1038/s41419-022-05368-6

Figure Lengend Snippet: Primer sequences of TLR1-10, IL-1β, IL-6, IL-12α, IL-12β, CXCL9-11 and GAPDH.

Article Snippet: The NF-κB inhibitor BAY 11-7082, TLR4 inhibitor Resatorvid, TLR5 inhibitor TH1020 and TLR8 inhibitor CU-CPT-8m were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques:

Journal: Cell Death & Disease

Article Title: PA-MSHA induces inflamed tumor microenvironment and sensitizes tumor to anti-PD-1 therapy

doi: 10.1038/s41419-022-05368-6

Figure Lengend Snippet: A Confirmation of NF-κB, SRC, STAT1 changes by Western-Blot. B – C NF-κB blockade in THP-1 cells suppressed expression of TNF-α and IL-6. D mRNA change of TLRs in THP-1 derived macrophages after treatment with PA-MSHA for 6 hours, 12 hours and 24 hours. E – F Inhibitor of TLR5 (TH1020, 5 μM) suppressed the expression of TNF-α and IL-6. P-M is short for PA-MSHA. (* P < 0.05, ** P < 0.01, *** P < 0.001, *** *P < 0.0001.).

Article Snippet: The NF-κB inhibitor BAY 11-7082, TLR4 inhibitor Resatorvid, TLR5 inhibitor TH1020 and TLR8 inhibitor CU-CPT-8m were purchased from MedChem Express (Monmouth Junction, NJ, USA).

Techniques: Western Blot, Expressing, Derivative Assay